Chloride intracellular channel gene knockdown induces insect cell lines death and level increases of intracellular calcium ions

Chloride intracellular channel (CLIC) is a member of the chloride protein family for which growing evidence supports pivotal role in fundamental cellular events. However, physiological function CLIC insects still rarely uncovered. The ovary-derived High Five (Hi-5) cell line isolated from cabbage looper ( Trichoplusia ni ) widely used laboratories. Here, we studied both characteristics and functions Hi-5 cells Tn CLIC). We identified TnCLIC gene annotated highly conserved proteins most insect species. After RNA interference , phenomenon significantly increased death suggests that essential survival cells. same lethal effect was also observed Spodoptera frugiperda 9 Drosophila melanogaster Schneider 2 after knockdown. Furthermore, found this kind accompanied by increases calcium ions knockdown with transcriptomic analyses detection levels. Our results provide insights into as key factor lay foundation mechanism.